Anti-beta Actin antibody - Loading Control

• Supplier: Abcam
• Catalog: ab8229
• Prices: $400.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Rat, Rabbit, Chicken, Bovine, Dog, Human, Fish, Monkey, Hamster, Broad species
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin
• Description: Goat Polyclonal
• Gene: ACTB
• Conjugate: Unconjugated

Product images

All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1/1000 dilutionLane 1 : HeLa whole cell (Human)Lane 2 : 3T3 cell (Mouse)Lane 3 : Rabbit LiverLane 4 : MDCK cell (Dog) Lane 5 : EBTr cell (Cow)Lane 6 : SL-29 cell (Chicken)Lane 7 : CHO cell (Chinese Hamster)Lysates/proteins at 20 µg per lane.SecondaryRabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab8229 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab8229, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

IHC image of beta Actin staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8229, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6885), donkey anti-goat HRP (IgG - H&L; 1/250 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Lanes 1 - 4 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 5% BSA.)Lanes 5 - 8 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 3% Milk.)Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 4 : CHO-K1 cell lysate Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 8 : CHO-K1 cell lysate Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

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