All lanes : Anti-beta B1 Crystallin antibody (ab54655) at 1/100 dilutionLane 1 : Zebrafish lens homogenate Lane 2 : Zebrafish lens homogenateLane 4 : Human Beta B1 crystallinLane 5 : Human Beta B2 crystallin (Negative Control)Lysates/proteins at 1 µg per lane.SecondaryAlkaline phosphatase-conjugated Goat anti-mouse IgG monoclonal at 1/3000 dilutionObserved band size : 45 kDa (why is the actual band size different from the predicted?)Additional bands at : 30 kDa,82 kDa. We are unsure as to the identity of these extra bands.This image is courtesy of an Abreview submitted by Dr. Leonel Trujillo See Abreview