Anti-beta Catenin (phospho Y142) antibody
| Name | Anti-beta Catenin (phospho Y142) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83295 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F |
| Species Reactivities | Rabbit, Human, Mouse, Horse, Chicken, Bovine, Pig, Xenopus, Drosophila, Primate, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human beta Catenin |
| Description | Rabbit Polyclonal |
| Gene | CTNNB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-beta Catenin (phospho Y142) antibody (ab83295) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human beta Catenin (phospho Y142) peptide (ab100819) at 1 µg/mlLane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Human beta Catenin (phospho Y142) peptide (ab100819) at 1 µg/mlLane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with Human beta Catenin (phospho Y142) peptide (ab100819) at 1 µg/mlLane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with beta Catenin peptide (ab116624) at 1 µg/mlLane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with beta Catenin peptide (ab116624) at 1 µg/mlLane 9 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate with beta Cateni
ICC/IF image of ab83295 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab83295 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.