Anti-beta Catenin antibody

Name	Anti-beta Catenin antibody
Supplier	Abcam
Catalog	ab16051
Prices	$400.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	IP IHC-P ICC/IF ICC/IF IHC-F ELISA WB
Species Reactivities	Mouse, Rat, Human, Xenopus, Zebrafish, Chicken, Pig
Antigen	Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human beta Catenin
Blocking Peptide	Human beta Catenin peptide
Description	Rabbit Polyclonal
Gene	CTNNB1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ab16051 staining β-Catenin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab16051 at 1μg/ml and ab7291 (staining Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

ab16051 staining β-catenin in SW480 cells treated with XAV939 (ab120897), by ICC/IF. Increase of ß-catenin cytoplasmic expression and decrease in nuclear expression correlates with increased concentration of XAV939, as described in literature.The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120897 (XAV939) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Standard Curve for Beta-Catenin (Analyte: beta Catenin protein (Tagged) (ab63175)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [BDI080] to beta Catenin (ab19448) at 1ug/ml and Detector Antibody Rabbit polyclonal to beta Catenin (ab16051) at 0.1ug/ml.

Performed under reducing conditions.

ICC/IF image of ab16051 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16051X, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

ab16051 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to incubation with the primary antibody (at 10µg/ml) for 1 hour at 27°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.See Abreview

ab16051 staining beta Catenin in rat hypothalamus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody (1/100) for 18 hours at 200C in PBS + 0.3 % Triton X100. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.See Abreview

developed using the ECL techniquePerformed under reducing conditions.

IHC image of beta Catenin staining in Human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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