Anti-beta Catenin antibody
| Name | Anti-beta Catenin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2365 |
| Prices | $398.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IHC-F ICC/IF ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Chicken, Human, Xenopus |
| Antigen | Synthetic peptide corresponding to Human beta Catenin aa 768-781 (C terminal) |
| Description | Rabbit Polyclonal |
| Gene | CTNNB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2365 at 1/100 staining rat gastric epithelial cells by ICC/IF. The cells were fomaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. A FITC conjugated goat anti-rabbit IgG was used as the secondary. Nuclei were counterstained with propidium iodide.The image shows the staining of gastric epithelial cells (left hand panel) and the staining of embyonic fibroblasts (negative control, right hand panel).See Abreview
ab2365 at 1/200 staining mouse skin tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit polyclonal was used as the secondary.See Abreview
Anti-beta Catenin antibody (ab2365) at 1/25 dilution + A431 cell lysate
![Beta Catenin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to beta Catenin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2365.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 85kDa; beta Catenin.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/13877_beta-Catenin-Primary-antibodies-ab2365-10.jpg)
Beta Catenin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to beta Catenin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2365.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 85kDa; beta Catenin.
Product References
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Ngamjariyawat A, Turpaev K, Vasylovska S, Kozlova EN, Welsh N. PLoS One. 2013 Apr 17;8(4):e61828.
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Semont A, Demarquay C, Bessout R, Durand C, Benderitter M, Mathieu N. PLoS One. 2013 Jul 29;8(7):e70170.
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Distler A, Deloch L, Huang J, Dees C, Lin NY, Palumbo-Zerr K, Beyer C, Weidemann A, Distler O, Schett G, Distler JH. Ann Rheum Dis. 2013 Sep 1;72(9):1575-80.
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Distler A, Ziemer C, Beyer C, Lin NY, Chen CW, Palumbo-Zerr K, Dees C, Weidemann A, Distler O, Schett G, Distler JH. Ann Rheum Dis. 2014 Mar;73(3):624-7.
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Maran RR, Thomas A, Roth M, Sheng Z, Esterly N, Pinson D, Gao X, Zhang Y, Ganapathy V, Gonzalez FJ, Guo GL. J Pharmacol Exp Ther. 2009 Feb;328(2):469-77.