All lanes : Anti-beta I Tubulin antibody (ab135838) at 1/100 dilutionLane 1 : CEM cell lysateLane 2 : MCF-7 cell lysateLane 3 : MDA-MB231 cell lysateLysates/proteins at 35 µg per lane.
immunohistochemical analysis of formalin-fixed and paraffin-embedded Human brain tissue labelling beta I Tubulin with ab135838 at 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram) labelling beta-I-Tubulin with ab135838 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.