Anti-beta II Tubulin antibody [EPR16773]

• Supplier: Abcam
• Catalog: ab179512
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16773
• Applications: WB IHC-P ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta II Tubulin aa 400 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: TUBB4B
• Conjugate: Unconjugated

Product images

Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/10000 dilution + Human fetal brain lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/1000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Mouse spleen lysateLane 5 : Rat brain lysateLane 6 : Rat heart lysateLane 7 : Rat kidney lysateLane 8 : C6 (Rat glial tumor cells) whole cell lysateLane 9 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling beta II Tubulin with ab179512 at 1/100 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows both cytoplasm and nuclear staining on the SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).The negative controls are as follows:--ve control 1: - ab179512 at 1/100 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.-ve control 2:. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/400 dilution.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of cytoplasmic and nuclear expression on neuron cells of Human cerebral cortex is observed. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human glioma labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on Human glioma cells is observed. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of expression on neuron cells of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of expression on neuron cells of Rat cerebral cortex is observed. Counter stained with Hematoxylin.Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling beta II Tubulin with ab179512 at 1/450 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Beta II Tubulin was immunoprecipitated from 1mg of Human fetal brain extract with ab179512 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab179512 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal brain extract. Lane 2: PBS instead of extract.Blocking/Dilution buffer: 5% NFDM/TBST.