Anti-Betacellulin antibody - Aminoterminal end (ab170516) at 1/100 dilution + MDA-MB231 cell lysate at 35 µg
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescence analysis of MDA-MB231 cells, labeling Betacellulin using ab170516 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Flow cytometry analysis of HeLa cells labeling Betacellulin (green, right histogram), using ab170516 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.