Anti-Bim antibody
| Name | Anti-Bim antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7888 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Peptide corresponding to amino acids 22 to 40 of human origin |
| Description | Rabbit Polyclonal |
| Gene | BCL2L11 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Bim antibody (ab7888) at 1 µg/mlLane 1 : K562 whole cell lysateLane 2 : A549 whole cell lysate
ab7888 at 20µg/ml staining Bim in human cancer cells by IHC-P
ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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