Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Bmf with ab181148 at 1/40 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
All lanes : Anti-Bmf antibody [EPR10930(2)] (ab181148) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : Ramos cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution