Anti-BMP2 antibody - Differentiation marker
| Name | Anti-BMP2 antibody - Differentiation marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab17885 |
| Prices | $379.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human |
| Antigen | Recombinant fragment corresponding to Human BMP2 aa 283-396 |
| Description | Rabbit Polyclonal |
| Gene | BMP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab17885 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-BMP2 antibody - Differentiation marker (ab17885) at 0.1 µg/ml + Human BMP2 full length protein (ab87065) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds