Anti-BNIP3 antibody
| Name | Anti-BNIP3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38621 |
| Prices | $390.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | KLH conjugated synthetic peptide, selected from within aa 1-100 (BH3 domain) of human NIP3 |
| Description | Rabbit Polyclonal |
| Gene | BNIP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-BNIP3 antibody (ab38621) at 1/100 dilutionLane 1 : Ramos cell lysateLane 2 : Mouse brain tissue lysate
This image shows human breast carcinoma stained with ab38621 at 1/50 dilution.
This image shows human colon carcinoma stained with ab38621 at 5ug/200ul dilution.
ICC/IF image of ab38621 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38621, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through - Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through
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Cyclosporine does not reduce myocardial infarct size in a porcine - Cyclosporine does not reduce myocardial infarct size in a porcine
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Absence of the Birt-Hogg-Dube gene product is associated with increased - Absence of the Birt-Hogg-Dube gene product is associated with increased
Preston RS, Philp A, Claessens T, Gijezen L, Dydensborg AB, Dunlop EA, Harper KT, Brinkhuizen T, Menko FH, Davies DM, Land SC, Pause A, Baar K, van Steensel MA, Tee AR. Oncogene. 2011 Mar 10;30(10):1159-73.
Fiber type conversion by PGC-1alpha activates lysosomal and autophagosomal - Fiber type conversion by PGC-1alpha activates lysosomal and autophagosomal
Takikita S, Schreiner C, Baum R, Xie T, Ralston E, Plotz PH, Raben N. PLoS One. 2010 Dec 13;5(12):e15239.
Hypoxia-induced alteration of mitochondrial genes in cardiomyocytes: role of - Hypoxia-induced alteration of mitochondrial genes in cardiomyocytes: role of
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