All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal kidney lysateLane 3 : Human ovary cancer lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : PC-3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution + Rat spleen lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of Bok in PC-3 cell lysate immunoprecipitated with ab186745 at 1/50 dilution (Lane1). Lane 2: PBS instead of PC-3 cell lysate.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.