Anti-Bone Sialoprotein antibody
| Name | Anti-Bone Sialoprotein antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33022 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB IHC-P |
| Species Reactivities | Human |
| Antigen | A region within synthetic peptide: SATTLGYGED ATPGTGYTGL AAIQLPKKAG DITNKATKEK ESDEEEEEEE, corresponding to N terminal amino acids 108-157 of Human Bone Sialoprotein Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | IBSP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Bone Sialoprotein antibody (ab33022) at 1.25 µg/ml + HepG2 cell lysate SecondaryHRP conjugated anti Rabbit IgG at 1/50000 dilution
IHC image of ab33022 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33022, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.