All lanes : Anti-Bora antibody [EPR11610] (ab182149) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling Bora with ab182149 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 diution.
Anti-Bora antibody [EPR11610] (ab182149) at 1/10000 dilution + Jurkat cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab182149 at 1/40 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.