Anti-Brachyury / Bry antibody - N-terminal
| Name | Anti-Brachyury / Bry antibody - N-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab156611 |
| Prices | $376.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to Human Brachyury/ Bry aa 15-43 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | T |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Brachyury / Bry antibody - N-terminal (ab156611) at 1/100 dilution + MDA-MB453 cell lysate at 35 µg
Immunofluorescence of Hela cells labelling Brachyury/Bry with ab156611. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab156611 (1/25, 1 h at 37°C). An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Brachyury/Bry immunoreactivity is localized to the nucleus.