Anti-BRD2 antibody [EPR7642]

• Supplier: Abcam
• Catalog: ab139690
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7642
• Applications: WB FC ICC/IF ICC/IF IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BRD2 aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: BRD2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab139690 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139690, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-BRD2 antibody [EPR7642] (ab139690) at 1/1000 dilutionLane 1 : MOLT4 cell lysateLane 2 : Jurkat cell lysateLane 3 : NCCIT cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique

Immunofluorescence analysis of HeLa cells labeling BRD2, with ab139690 at 1/250 dilution.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD2 with ab139690 at 1/250 dilution.