Anti-Brd4 antibody
| Name | Anti-Brd4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75898 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB |
| Species Reactivities | Mouse, Rat, Human, Horse, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human Brd4 |
| Description | Rabbit Polyclonal |
| Gene | BRD4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab75898 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/mg lysate in 50mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 (1/10000) was used to confirm successful immunoprecipation.Detects band around 150 kDa (predicted size) also additional band around 250 kDa (possibly modified Brd4). Secondary antibody - anti-mouse HRP (ab131366)See Abreview
All lanes : Anti-Brd4 antibody (ab75898) at 1 µg/mlLane 1 : Liver (Mouse) Tissue Lysate Lane 2 : Liver (Rat) Tissue Lysate Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg/ml per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) developed using the ECL techniquePerformed under reducing conditions.
ab75898 (1/500) staining Brd4 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview
ICC/IF image of ab75898 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75898, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.
Product References
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Liu P, Xiang Y, Fujinaga K, Bartholomeeusen K, Nilson KA, Price DH, Peterlin BM. J Biol Chem. 2014 Apr 4;289(14):9918-25.
The bromodomain protein BRD4 regulates the KEAP1/NRF2-dependent oxidative stress - The bromodomain protein BRD4 regulates the KEAP1/NRF2-dependent oxidative stress
Hussong M, Borno ST, Kerick M, Wunderlich A, Franz A, Sultmann H, Timmermann B, Lehrach H, Hirsch-Kauffmann M, Schweiger MR. Cell Death Dis. 2014 Apr 24;5:e1195.
Targeting MYCN in neuroblastoma by BET bromodomain inhibition. - Targeting MYCN in neuroblastoma by BET bromodomain inhibition.
Puissant A, Frumm SM, Alexe G, Bassil CF, Qi J, Chanthery YH, Nekritz EA, Zeid R, Gustafson WC, Greninger P, Garnett MJ, McDermott U, Benes CH, Kung AL, Weiss WA, Bradner JE, Stegmaier K. Cancer Discov. 2013 Mar;3(3):308-23.
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Bartholomeeusen K, Xiang Y, Fujinaga K, Peterlin BM. J Biol Chem. 2012 Oct 19;287(43):36609-16.