Anti-Breast cancer suppressor candidate 1 antibody
| Name | Anti-Breast cancer suppressor candidate 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64977 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide from internal sequence of human Breast cancer suppressor candidate 1 |
| Description | Rabbit Polyclonal |
| Gene | VWA5A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Breast cancer suppressor candidate 1 antibody (ab64977) at 1/500 dilutionLane 1 : extracts from 293 cells (5-30µg total protein)Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.
ICC/IF image of ab64977 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64977, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.