Anti-BRIT1 antibody
| Name | Anti-BRIT1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2612 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to Human BRIT1 aa 400-500 |
| Blocking Peptide | Human BRIT1 peptide |
| Description | Rabbit Polyclonal |
| Gene | MCPH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lanes 1 - 3 : Anti-BRIT1 antibody (ab2612) at 1/250 dilutionLanes 4 - 6 : Anti-BRIT1 antibody (ab2612) at 1/500 dilutionLanes 7 - 9 : Anti-BRIT1 antibody (ab2612) at 1/1000 dilutionLane 1 : 30 ug of LCL1 whole cell lysate.Lane 2 : 30 ug of LCL2 whole cell lysate.Lane 3 : 30 ug of LCL3 whole cell lysate.Lane 4 : 30 ug of LCL1 whole cell lysate.Lane 5 : 30 ug of LCL2 whole cell lysate.Lane 6 : 30 ug of LCL3 whole cell lysate.Lane 7 : 30 ug of LCL1 whole cell lysate.Lane 8 : 30 ug of LCL2 whole cell lysate.Lane 9 : 30 ug of LCL3 whole cell lysate.Performed under reducing conditions.
All lanes : Anti-BRIT1 antibody (ab2612) at 1/1000 dilutionLane 1 : GUS (beta glucuronidase) expression negative control. Lane 2 : In vitro expressed human BRIT1SecondaryA rabbit polyclonal to Goat IgG H&L (HRP) secondary antibody was used at a 1:5000 dilution.developed using the ECL techniquePerformed under reducing conditions.
Image courtesy of Human Protein Atlas ab2612 staining in human lung, showing staining of the surface epithelial cells (in brown). Paraffin embedded lung tissue was incubated with ab2612 (1:100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab2612 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
Immunohistochemistical detection of BRIT1 using antibody (ab2612) on paraformaldehyde perfusion fixed mouse heart tissue sections. Primary antibody diluted @ 1/300 & incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit conjugated to biotin (1/300). The antibody produced a weak cytoplasmic staining in cardiac cells.See Abreview
All lanes : Anti-BRIT1 antibody (ab2612) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human BRIT1 peptide (ab13737) at 1 µg/mlLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human BRIT1 peptide (ab13737) at 1 µg/mlLane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human BRIT1 peptide (ab13737) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
All lanes : Anti-BRIT1 antibody (ab2612) at 1 µg/ml (BLOCKED IN 5% MILK)Lane 1 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml) Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 1uM) Lysates/proteins at 25 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutionPerformed under reducing conditions.
Product References
Deregulation of microcephalin and ASPM expression are correlated with epithelial - Deregulation of microcephalin and ASPM expression are correlated with epithelial
Alsiary R, Bruning-Richardson A, Bond J, Morrison EE, Wilkinson N, Bell SM. PLoS One. 2014 May 15;9(5):e97059.
Mcph1-deficient mice reveal a role for MCPH1 in otitis media. - Mcph1-deficient mice reveal a role for MCPH1 in otitis media.
Chen J, Ingham N, Clare S, Raisen C, Vancollie VE, Ismail O, McIntyre RE, Tsang SH, Mahajan VB, Dougan G, Adams DJ, White JK, Steel KP. PLoS One. 2013;8(3):e58156.
Primary microcephaly gene MCPH1 shows signatures of tumor suppressors and is - Primary microcephaly gene MCPH1 shows signatures of tumor suppressors and is
Venkatesh T, Nagashri MN, Swamy SS, Mohiyuddin SM, Gopinath KS, Kumar A. PLoS One. 2013;8(3):e54643.
A high-throughput assay to identify modifiers of premature chromosome - A high-throughput assay to identify modifiers of premature chromosome
Adams M, Cookson VJ, Higgins J, Martin HL, Tomlinson DC, Bond J, Morrison EE, Bell SM. J Biomol Screen. 2014 Jan;19(1):176-83.
MCPH1 functions in an H2AX-dependent but MDC1-independent pathway in response to - MCPH1 functions in an H2AX-dependent but MDC1-independent pathway in response to
Wood JL, Singh N, Mer G, Chen J. J Biol Chem. 2007 Nov 30;282(48):35416-23. Epub 2007 Oct 9.
Microcephalin is a DNA damage response protein involved in regulation of CHK1 and - Microcephalin is a DNA damage response protein involved in regulation of CHK1 and
Xu X, Lee J, Stern DF. J Biol Chem. 2004 Aug 13;279(33):34091-4. Epub 2004 Jun 25.