Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122174 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122174, at 1/500 dilution, staining BROX in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
All lanes : Anti-BROX antibody (ab122174) at 1/250 dilutionLane 1 : RT 4 lysateLane 2 : U 251 MG lysateLane 3 : Human plasma lysateLane 4 : Human liver lysateLane 5 : Human tonsil lysatedeveloped using the ECL technique