All lanes : Anti-BTF antibody [EPR9980(2)] (ab181240) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : MCF-7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BTF with ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling BTF with ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells labeling BTF with ab181240 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).