Anti-Bub1 antibody

• Supplier: Abcam
• Catalog: ab9000
• Prices: $382.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Rat, Human
• Antigen: His-tagged N-terminal bacterially expressed human Bub1 (aa 1-312)
• Description: Rabbit Polyclonal
• Gene: BUB1
• Conjugate: Unconjugated

Product images

ab9000 staining Bub1 in HCT cells by Immunocytochemistry/ Immunofluorescence. Cells were plated on 12 mm coverslips, pre-extracted with 0.2% Triton X-100 in warm PEM (100 mM PIPES pH6.8, 1mM MgCl2 and 5mM EGTA) for 1 minute, fixed with 3% PFA in PBS, blocked with 3% BSA in PBS for 1 hour, incubated with primary antibody for 16 hrs at 4°C, washed with PBS/0.1% Triton X-100 and incubated with secondary antibody for an additional 1 hour at room temperature. ACA staining in red, Bub1 is green and DNA (DAPI) is in blue.

ab9000, staining Bub1 in oocytes from Sprague-Dawley rats. Oocytes were fixed in paraformaldehyde, permeabilized with 0.5% Triton-X 100 in PHEM and blocked in PHEM containing 3% BSA for 1 hour. Samples were incubated with primary antibody at 1/500 dilution overnight at 4°C. A Cy3-conjugated goat-anti-rabbit IgG (1/800) was used as the secondary antibody.

Product References

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Maternal-restraint stress increases oocyte aneuploidy by impairing metaphase I - Maternal-restraint stress increases oocyte aneuploidy by impairing metaphase I

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Structural analysis reveals features of the spindle checkpoint kinase - Structural analysis reveals features of the spindle checkpoint kinase

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Chemical genetic inhibition of Mps1 in stable human cell lines reveals novel - Chemical genetic inhibition of Mps1 in stable human cell lines reveals novel

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Dissecting the role of MPS1 in chromosome biorientation and the spindle - Dissecting the role of MPS1 in chromosome biorientation and the spindle

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Genome-wide impact of androgen receptor trapped clone-27 loss on - Genome-wide impact of androgen receptor trapped clone-27 loss on

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The human spindle assembly checkpoint protein Bub3 is required for the - The human spindle assembly checkpoint protein Bub3 is required for the

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