Anti-Bub1 antibody
| Name | Anti-Bub1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54893 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 1-130 of Human Bub1 |
| Description | Mouse Monoclonal |
| Gene | BUB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab54893 (1/400) staining Bub1 in Assynchronous Hela cells (green). Cells were fixed in methanol and counterstained with DAPI (red). Please refer to Abreview for further experimental details.See Abreview
Anti-Bub1 antibody (ab54893) at 2.5 µg/ml + HeLa nuclear Lysate(20µg)
![Overlay histogram showing HeLa cells stained with ab54893 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54893, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/15990_Bub1-Primary-antibodies-ab54893-1.jpg)
Overlay histogram showing HeLa cells stained with ab54893 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54893, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry - Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry
Dunsch AK, Hammond D, Lloyd J, Schermelleh L, Gruneberg U, Barr FA. J Cell Biol. 2012 Sep 17;198(6):1039-54.
Transcriptional intermediary factor 1gamma binds to the anaphase-promoting - Transcriptional intermediary factor 1gamma binds to the anaphase-promoting
Sedgwick GG, Townsend K, Martin A, Shimwell NJ, Grand RJ, Stewart GS, Nilsson J, Turnell AS. Oncogene. 2013 Sep 26;32(39):4622-33.
Constitutive Mad1 targeting to kinetochores uncouples checkpoint signalling from - Constitutive Mad1 targeting to kinetochores uncouples checkpoint signalling from
Maldonado M, Kapoor TM. Nat Cell Biol. 2011 Apr;13(4):475-82.