Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Bub1 with ab70372 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-Bub1 antibody (ab70372) at 0.1 µg/mlLane 1 : HeLa whole cell lysate - asynchronously growingLane 2 : HeLa whole cell lysate - treated with NocodozaleLysates/proteins at 50 µg per lane.
Detection of Human Bub1 by Immunoprecipitiaton. HeLa Whole cell lysate used at 50 µg for WB Input; 1 mg for IP. Cells were asynchronously growing (-) or treated with Nocodozale (Noc +). ab70372 was used at 1 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection .