Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling BXDC1 with ab180604 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution (left panel). Dapi stain (right panel).
Immunohistochemical analysis of paraffin-embedded Human kidney cell carcinoma labeling BXDC1 with ab180604 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-BXDC1 antibody [EP10252] (ab180604) at 1/20000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution