Anti-C Peptide antibody [1H8]
| Name | Anti-C Peptide antibody [1H8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab8297 |
| Prices | $379.00 |
| Sizes | 250 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1H8 |
| Applications | ICC/IF ICC/IF IHC-P ELISA ELISA RIA |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein |
| Description | Mouse Monoclonal |
| Gene | INS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab8297 (1µg/ml) staining C Peptide in human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the cells of the islets of Langerhans.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab8297 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8297, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Evidence for an interaction between proinsulin C-peptide and GPR146. - Evidence for an interaction between proinsulin C-peptide and GPR146.
Yosten GL, Kolar GR, Redlinger LJ, Samson WK. J Endocrinol. 2013 Jul 11;218(2):B1-8.
At pharmacologically relevant concentrations intravenous iron preparations cause - At pharmacologically relevant concentrations intravenous iron preparations cause
Masuda Y, Ichii H, Vaziri ND. Am J Transl Res. 2013 Dec 1;6(1):64-70. eCollection 2013.