Anti-c-IAP1 and c-IAP2 antibody

• Supplier: Abcam
• Catalog: ab25939
• Prices: $370.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1
• Blocking Peptide: Human cIAP1 peptide
• Description: Rabbit Polyclonal
• Conjugate: Unconjugated

Product images

Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/mlLane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/mlLane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/mlLane 1 : Human lung lysateLane 2 : Human lung lysateLane 3 : Human lung lysate

ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC 

ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.

Product References

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Gualco E, Urbanska K, Perez-Liz G, Sweet T, Peruzzi F, Reiss K, Del Valle L. Cell Death Differ. 2010 Mar;17(3):439-51.

hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of - hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of

Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. Cell Death Differ. 2009 Feb;16(2):244-52.