Detection of Gi24/VISTA/B7‑H5 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Integrin alpha M/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P) and either (A) Mouse IgG2B Isotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human Gi24/VISTA/B7‑H5 Monoclonal Antibody (Catalog # MAB71261) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Gi24/VISTA/B7‑H5 in BG01V Human Embryonic Stem Cells. Gi24/VISTA/B7‑H5 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human Gi24/VISTA/B7‑H5 Monoclonal Antibody (Catalog # MAB71261) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.