Human CLEC14A Antibody
| Name | Human CLEC14A Antibody |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF4968 |
| Prices | $99.00, $359.00 |
| Sizes | 25 µg, 100 µg |
| Host | Sheep |
| Isotype | IgG |
| Applications | FC ICC/IF IHC WB |
| Species Reactivities | Human |
| Antigen | Mouse myeloma cell line NS0-derived recombinant human CLEC14A Glu22-Ala397 Accession # Q86T13 |
| Purity/Format | Antigen Affinity-purified |
| Description | Sheep |
| Gene | CLEC14A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Clec-14A in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968, filled histogram) or isotype control antibody (Catalog # 5-001-A , open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126 ).
CLEC14A in HUVEC Human Cells. CLEC14A was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010 ) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .
CLEC14A in Human Breast Cancer Tissue. CLEC14A was detected in formalin fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968) at 1.7 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Universal (Catalog # CTS015 ). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
Product References
Human antibodies targeting the C-type lectin-like domain of the tumor endothelial - Human antibodies targeting the C-type lectin-like domain of the tumor endothelial
Ki MK, Jeoung MH, Choi JR, Rho SS, Kwon YG, Shim H, Chung J, Hong HJ, Song BD, Lee S. Oncogene. 2013 Nov 28;32(48):5449-57.
Identification and angiogenic role of the novel tumor endothelial marker CLEC14A. - Identification and angiogenic role of the novel tumor endothelial marker CLEC14A.
Mura M, Swain RK, Zhuang X, Vorschmitt H, Reynolds G, Durant S, Beesley JF, Herbert JM, Sheldon H, Andre M, Sanderson S, Glen K, Luu NT, McGettrick HM, Antczak P, Falciani F, Nash GB, Nagy ZS, Bicknell R. Oncogene. 2012 Jan 19;31(3):293-305.