Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1 : Lanes 2 - 3 : Anti-C10orf53 antibody (ab122426)Lane 1 : MarkerLane 2 : HEK293T cell lysate - vector only transfectedLane 3 : HEK293T cell lysate - overexpressing C10orf53 with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling C10orf53 with ab122426. Staining shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.