Human Ly6K Antibody
| Name | Human Ly6K Antibody |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF6648 |
| Prices | $99.00, $359.00 |
| Sizes | 25 µg, 100 µg |
| Host | Sheep |
| Isotype | IgG |
| Applications | IHC WB |
| Species Reactivities | Human |
| Antigen | Chinese hamster ovary cell line CHO-derived recombinant human Ly6K Asp18-Gly138 Accession # Q17RY6 |
| Purity/Format | Antigen Affinity-purified |
| Description | Sheep |
| Gene | LY6K |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human Ly6K by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 0.25 µg/mL of Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). Specific bands were detected for Ly6K at approximately 25-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
Ly6K in Human Lung Cancer Tissue. Ly6K was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .