Detection of Human/Mouse MYCL1/L‑Myc by Western Blot. Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line, A549 human lung carcinoma cell line, JEG‑3 human epithelial choriocarcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MYCL1/L‑Myc at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
MYCL1/L‑Myc in HeLa Human Cell Line. MYCL1/L‑Myc was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, blue panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MYCL1/L‑Myc in NIH3T3 Mouse Cell Line. MYCL1/L‑Myc was detected in immersion fixed NIH3T3 mouse embryonic fibroblast cell line using Goat Anti-Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.