Detection of Mouse Integrin a11 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Integrin a11 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Integrin alpha 11 in Mouse Embryonic Fibroblasts (iMEF). Integrin alpha 11 was detected in immersion fixed irradiated mouse embryonic fibroblasts (iMEF) using Mouse Integrin alpha 11 Affinity-purified Polyclonal Antibody (Catalog # AF6498) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010 ) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .