Immunofluorescence analysis of U87-MG cells labeling C14orf166 using ab188326 at 1/100 dilution. A goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Immunoprecipitation analysis of 293T cell lysate labeling C14orf166 using ab188326 at 1/50 dilution (Lane 1). An anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of 293T cell lysate.
All lanes : Anti-C14orf166 antibody [EPR15799] (ab188326) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-C14orf166 antibody [EPR15799] (ab188326) at 1/10000 dilutionLane 1 : Ramos cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution