Anti-C14ORF21 antibody
| Name | Anti-C14ORF21 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab86119 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Rabbit, Bovine |
| Antigen | Synthetic peptide, corresponding to a region within internal sequence amino acids 215-264 (GTILESERAR PRGSQSSEAQ KTPAQECKPA DFEVPETFLN RLQDLSSSFL) of Human C14ORF21, (NP_777573) |
| Description | Rabbit Polyclonal |
| Gene | NOP9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-C14ORF21 antibody (ab86119) at 1 µg/ml + Hela cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab86119 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86119 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.