Detection of Mouse Wnt‑11 by Western Blot. Western blot shows lysates of mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt‑11 Monoclonal Antibody (Catalog # MAB3746) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt‑11 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Wnt‑11 in Mouse Embryo. Wnt‑11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Wnt‑11 Monoclonal Antibody (Catalog # MAB3746) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.