Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling C19orf63 with ab181209 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunofluorescent analysis of Ramos cells (-20°C Acetone-fixed) labeling C19orf63 with ab181209 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
All lanes : Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilutionLane 1 : 293 cell lysateLane 2 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilution + A549 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution