Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab150874 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling C19orf75 with ab150874 at 1/200 dilution.
Lane 1 : Anti-C19orf75 antibody (ab150874) at 1/100 dilutionLane 2 : Anti-C19orf75 antibody (ab150874) at 1/0 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : C19orf75 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells