Detection of Human and Rat FKBP13 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Monoclonal Antibody (Catalog # MAB4356) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .