Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli. Recommended concentration of ab121993 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-C1orf105 antibody (ab121993) at 1/250 dilutionLane 1 : Negative control (vector-only transfected HEK293T lysate) Lane 2 : C1orf105-transfected HEK293T overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))developed using the ECL technique
ab121993, at 1/400, staining C1orf105 in paraffin-embedded Human kidney by Immunohistochemistry.