TAFA5/FAM19A5 in Human Hypothalamus. TAFA5/FAM19A5 was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Human TAFA5/FAM19A5 Monoclonal Antibody (Catalog # MAB5148) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002 ) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .