Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab121891 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121891 at 1/800 dilution staining C1orf21 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
All lanes : Anti-C1orf21 antibody (ab121891) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)