Mouse Endomucin Antibody
| Name | Mouse Endomucin Antibody |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF4666 |
| Prices | $99.00, $359.00 |
| Sizes | 25 µg, 100 µg |
| Host | Goat |
| Isotype | IgG |
| Applications | FC ICC/IF WB |
| Species Reactivities | Mouse |
| Antigen | E. coli -derived recombinant mouse Endomucin Glu21-Glu90 Accession # Q9R0H2 |
| Purity/Format | Antigen Affinity-purified |
| Description | Goat |
| Gene | EMCN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Endomucin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # AB-108-C , open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108 ).
Endomucin in bEnd.3 Mouse Endothelioma Cell Line. Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003 ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .
Detection of Mouse Endomucin by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
Product References
Suppression of ischemia in arterial occlusive disease by JNK-promoted native - Suppression of ischemia in arterial occlusive disease by JNK-promoted native
Ramo K, Sugamura K, Craige S, Keaney JF, Davis RJ. Elife. 2016 Aug 9;5. pii: e18414.
Sox17 is required for normal pulmonary vascular morphogenesis. - Sox17 is required for normal pulmonary vascular morphogenesis.
Lange AW, Haitchi HM, LeCras TD, Sridharan A, Xu Y, Wert SE, James J, Udell N, Thurner PJ, Whitsett JA. Dev Biol. 2014 Mar 1;387(1):109-20.