All lanes : Anti-C1orf57 antibody [EPR14325-50] - N-terminal (ab182155) at 1/20000 dilutionLane 1 : U87-MG cell lysateLane 2 : Jurkat cell lysateLane 3 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescence analysis of A549 cells, staining C1orf57 (green) with ab182155 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of U87-MG cells, staining C1orf57 (red) with ab182155 at 1/100 dilution. An Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate (lane 1) or negative control (lane 2), labeling C1orf57 immunoprecipitated using ab182155 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.