Anti-C1q antibody [9A7]

• Supplier: Abcam
• Catalog: ab71089
• Prices: $388.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 9A7
• Applications: WB ELISA IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: C1q protein purified from Human plasma
• Description: Mouse Monoclonal
• Gene: C1QA
• Conjugate: Unconjugated

Product images

All lanes : Anti-C1q antibody [9A7] (ab71089) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : Jurkat T cell lysateLane 3 : C6 cell lysateLane 4 : NIH3T3 cell lysate

ab71089 (1µg/ml) staining C1q in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of of the epithelium.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Product References

Complement expression in the retina is not influenced by short-term pressure - Complement expression in the retina is not influenced by short-term pressure

Astafurov K, Dong CQ, Panagis L, Kamthan G, Ren L, Rozenboym A, Perera TD, Coplan JD, Danias J. Mol Vis. 2014 Jan 31;20:140-52. eCollection 2014.

Efficacy of vitamin D in treating multiple sclerosis-like neuroinflammation - Efficacy of vitamin D in treating multiple sclerosis-like neuroinflammation

Adzemovic MZ, Zeitelhofer M, Hochmeister S, Gustafsson SA, Jagodic M. Exp Neurol. 2013 Nov;249:39-48.