Human ADAMTS15 Antibody
| Name | Human ADAMTS15 Antibody |
|---|---|
| Supplier | R&D Systems |
| Catalog | MAB5149 |
| Prices | $99.00, $249.00 |
| Sizes | 25 µg, 100 µg |
| Host | Mouse |
| Isotype | IgG 2B |
| Clone | 561819 |
| Applications | FC IHC WB |
| Species Reactivities | Human |
| Antigen | Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS15 Gly18-Cys682 Accession # Q8TE58 |
| Purity/Format | Protein A or G purified from hybridoma culture supernatant |
| Description | Mouse |
| Gene | ADAMTS15 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human ADAMTS15 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for mature ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
Detection of ADAMTS15 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149, filled histogram) or isotype control antibody (Catalog # MAB0041 , open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0102B ).
ADAMTS15 in Human Kidney. ADAMTS15 was detected in immersion fixed paraffin-embedded sections of human kidney using Human ADAMTS15 Monoclonal Antibody (Catalog # MAB5149) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002 ) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .