Detection of Mouse IFN-? by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IFN-? Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for IFN-? at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Detection of Mouse IFN-? by Simple Western TM . Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and EL-4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for IFN-? at approximately 40 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse IFN-? Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.