Detection of Human IGSF3 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGSF3 Monoclonal Antibody (Catalog # MAB4788) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for IGSF3 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1 .
Detection of IGSF3 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human IGSF3 Monoclonal Antibody (Catalog # MAB4788, filled histogram) or isotype control antibody (Catalog # MAB002 , open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0102B ).