Anti-C20orf7 antibody [EPR15870] - C-terminal

• Supplier: Abcam
• Catalog: ab192235
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15870
• Applications: IHC-P ICC/IF ICC/IF IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human C20orf7 aa 200 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: NDUFAF5
• Conjugate: Unconjugated

Product images

All lanes : Anti-C20orf7 antibody [EPR15870] - C-terminal (ab192235) at 1/5000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293 cell lysateLane 3 : HeLa cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-C20orf7 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilutionLane 1 : Human skeletal muscle lysateLane 2 : Human fetal liver lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-C20orf7 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Mouse spleen lysateLane 5 : RAW 264.7 lysateLane 6 : NIH 3T3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling C20orf7 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling C20orf7 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunofluorescent analysis of NIH 3T3 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling C20orf7 with ab192235 at 1/400 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).Negative controls: anti-C20orf7 at 1/400 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

Western blot analysis of pellet from 293 cell lysate immunoprecipitated using ab192235 at 1/100 dilution (lane 1) or PBS control (lane 2).Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.